WebAug 14, 2024 · (A) CHX-dependent hyperphosphorylation of FRQ is impaired in a prd-4 knockout strain. Liquid cultures of WT and ∆prd-4 strains were grown in constant light. Mycelia were harvested before and 2 h after addition of CHX. Western blots were decorated with antibodies against FRQ. (B) PRD-4 is active in extracts from cells … WebJan 17, 2008 · Western blot result showed that both human and murine IFN-β productions of Hela cells and MCA38 cells were mildly induced after 9 h of poly I:C/CHX treatment (Fig 5A). Neutralization of IFN-β with …
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WebApr 30, 2014 · Western blot, RNAi and CCK-8 assays were used to investigate the relationship between Mcl-1 upregulation and ABT-263 sensitivity in HCC cells. Real-time PCR and Western blot were used to detect Mcl-1 mRNA and protein levels. Luciferase reporter assay and RNA synthesis inhibition assay were adopted to analyze the … WebJan 1, 2015 · 100 mg/ml cycloheximide (CHX) stock solution (Sigma-Aldrich, catalog … flow images
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WebJan 5, 2015 · Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational elongation. It is broadly used in cell biology in terms of determining the half-life of a given protein and has gained much pop … WebWestern blot analysis of extracts from Jurkat cells, untreated (-) or treated with increasing concentrations of Cycloheximide (24 hr), using PARP … WebJul 23, 2024 · This study aims to investigate the biological role of 6-methyladenine (m6A) methylation in inducing the carcinogenesis of glioma and its proliferation. Relative levels of ALKBH5 and glucose-6-phosphate dehydrogenase (G6PD) in glioma tissues and cell lines were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and … green care services pte ltd