Cigarette smoke extract experiments
Accumulated evidence shows that cigarette smoking is a key risk factor for COPD, and cigarette smoke extract (CSE), which contains more than 4000 different types of constituents, including many toxic oxidants, is capable of inducing oxidative stress in airway epithelial cells, and ultimately, damaging … See more To demonstrate the effect of cigarette smoking on the changes of SUMOylated proteins in HBEs, we examined the expression of SUMO proteins and SUMOylation associated enzymes following CSE … See more Next, we sought to conduct comparative proteomic analysis of SUMOylated substrates between CSE insulted and control HBEs. For this purpose, HBEs were treated with … See more KEGG significant enrichment was then conducted to identify the biochemical metabolic pathways of differentially SUMOylated substrates. KEGG analysis revealed 14 … See more The above characterized substrates were further examined by GO analysis and categorized according to their cellular localization. Of note, the most abundant proteins were derived from the nucleus (37%), followed by … See more WebCSE was prepared using a modification of a previously described method 16, 35 to increase reproducibility amongst experiments. Briefly, this was done by bubbling cigarette smoke through 1 mL basal medium, per cigarette (3R4F, University of Kentucky), followed by passage through a 0.45 µM filter for sterilization.
Cigarette smoke extract experiments
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WebJul 28, 2024 · Cigarette smoke extract (CSE) induced ER stress in J774 macrophages and RhoA activation in J774 cells, and the CSE-induced ROCK activity was successfully reversed by GSK2606414 and... WebTo Study the Toxic Effects of Cigarette Smoke - Week 2 During this laboratory period you will conduct the experiment you designed last week. Organize the members of your …
WebJul 6, 2024 · A study of the effect of cigarette smoke extract (CSE) on lung lining cells clarifies the interaction between CSE and an enzyme implicated in causing chronic obstructive pulmonary disease (COPD ... WebNov 6, 2024 · Cigarette smoke extract (CSE) was prepared as described below. In short, two non-filtered cigarettes (containing 0.8 mg of nicotine and 10 mg of tar per cigarette, Shishi, China) were burned with a vacuum pump, and then smoke was bubbled through the 20 ml serum-free medium to collect CSE.
WebNov 14, 2012 · Cigarette smoke extract induced mRNA expression of Apo J, CTGF, and fibronectin. The mRNA expressions of apolipoprotein J (Apo J), connective tissue growth … WebIn this study, we demonstrate how a mixture of plant sterols (MPtS) consisting of β-sitosterol, campesterol and stigmasterol, at serum concentration reached after ingestion of a drink enriched with plant sterols, inhibits eryptosis induced by cigarette smoke extract (CSE). Isolated RBCs were exposed for 4 h to CSE (10–20% v/v).
WebHello, I'm doing a smoking study on BEAS-2B cells, and was wondering what the most appropriate method of preparing cigarette smoke extract in the lab, I do not have access to a smoke machine. Also ...
WebUnder a cigarette smoke extract (CSE) stimulation condition, the apoptosis was analyzed by flow cytometry assay. Caspase-3 activity was examined with a caspase-3 activity assay kit; besides, inflammation and oxidative stress were assessed by measuring interleukin-6, tumor necrosis factor-α, glutathione/oxidized glutathione, reactive oxygen ... rcat earningsWebThe experiment was conducted using an in situ Cell Death Detection kit (11684817910, Roche, ... Chen H, Liao K, Cui-Zhao L, et al. Cigarette smoke extract induces apoptosis of rat alveolar type II cells via the PLTP/TGF-beta1/Smad2 pathway. Int … sims 4 male baggy pants ccWebAug 12, 2024 · Electronic cigarette extract (ECE) was prepared by bubbling smoke from the electronic cigarette using liquid that contains 6 mg of nicotine per ml through 15 mL of deionized water at a speed of 60 cc/min for 10 min. Cigarette smoke extract (CSE) was prepared from one 84-mm cigarette (research cigarette 3R4F; University of Kentucky) … sims 4 male baggy sweaterhttp://www.tobaccoinduceddiseases.org/Cigarette-smoke-extract-affects-methylation-status-and-nattenuates-Sca-1-expression,131625,0,2.html rcat earnings reportWebJan 9, 2024 · For the smoke exposure experiments, cigarette smoke extract (CSE) was prepared as previously described . Beas2b cells were treated with cysteamine and/or CSE at various concentrations for 6 h or overnight. Western blotting. Caspase-3/7, DNA fragmentation, and senescence assays. rcat earnings dateWebNov 1, 2024 · A comparative study of a HNB cigarette (maximum heating temperature of 350 °C) and a combustion cigarette has shown that the low operating temperature of … sims 4 male athletic wearWebIn this study, we demonstrate how a mixture of plant sterols (MPtS) consisting of β-sitosterol, campesterol and stigmasterol, at serum concentration reached after ingestion of a drink … sims 4 male baggy shirts