Phospho 4ebp1 antibody

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August undefined, 2024

WebAll lanes : Anti-eIF4EBP1 (phospho S65) antibody [EPR23889-3] at 1/1000 dilution Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate Lane 2 : HeLa treated with 100nM rapamycin for 24 hours, whole cell whole cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at … WebAnti-eIF4EBP1 (phospho T36) antibody See all eIF4EBP1 primary antibodies Description Rabbit polyclonal to eIF4EBP1 (phospho T36) Host species Rabbit Specificity ab47365 detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.

Phospho-4EBP1 (Thr37, Thr46) Polyclonal Antibody

WebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health Analytical & Enterprise Solutions An Analytical and Enterprise solutions business, including leading OneSource laboratory services, focused on accelerating scientific outcomes Webp-4E-BP1 Antibody (62.Ser 65): sc-293124. Western blot analysis of 4E-BP1 phosphorylation in HeLa (A), K-562 (B) and HEL 92.1.7 (C) whole cell lysates. Description Gene Info Datasheets and Protocols The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5′ end of mRNA. simple homemade thousand island dressing

Recombinant Anti-eIF4EBP1 (phospho S65) antibody …

WebThe Simple Western TM antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. WebThe lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -2℃ . When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. 溶质携带物家族-1抗体， ... WebListed below are anti-4EBP1 antibodies from multiple suppliers. 4EBP1 is a reported alias name for the human gene EIF4EBP1, or 'eukaryotic translation initiation factor 4E binding protein 1'. ... Immunotag™ Phospho-4EBP1(Ser65/Thr70) Antibody. Applications: WB, IHC; Reactivity: Hu, Ms, Rt; Conjugate/Tag: Unconjugated, Biotin, FITC, HRP, Alexa ... raw material colour code chart

p-4E-BP1 Antibody (62.Ser 65) SCBT - Santa Cruz Biotechnology

Rapamycin differentially inhibits S6Ks and 4E-BP1 to mediate ... - PNAS

Web实验用到的供体是Thr46（铽标记的anti-phospho-4EBP1），受体是绿色荧光标记的4EBP1，是mTOR下游信号通路的蛋白，有活性的mTOR能磷酸化4EBP1，使之与供体结合。如果mTOR活性受到抑制，供体受体距离不能达到荧光能量转移的要求，则在515nm处与485nm处的信号比值增大。 WebOur 4EBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. Use the list below to choose the 4EBP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. raw material cost increase 2021WebThis antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1. Tested applications Suitable for: IHC-P, WB more details Species reactivity Reacts with: Rat, Human Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan Immunogen raw material companies to invest in

"WebMar 30, 2016 · When 4EBP1, an eIF4E-binding protein, is active (non-p-4EBP1), it binds to eIF4E and hinder formation of the initiation complex and then translation is blocked, favoring apoptosis.... " - Phospho 4ebp1 antibody

Phospho 4ebp1 antibody

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855

WebProduct Description. Rabbit anti 4EBP1 antibody recognizes an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation. The association between 4EPB1 and eukaryotic ... WebInvitrogen Anti-Phospho-4EBP1 (Thr37, Thr46) Polyclonal, Catalog # PA5-114544. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (PFA fixed) (IHC (PFA)) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 100 µL purified antibody (1 mg/mL).

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WebMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser65 of mouse 4E-BP1. Background Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. WebMA Beigh's Publication in Biomed Res Int....Sigma unless otherwise indicated. Anti-S6K antibody (Santa Cruz Biotechnologies Inc), Anti-S6K (Polyclonal-Genscript), Anti-Erk1, Anti-phospho-Erk, Anti-4EBP1, Anti-phospho- 4EBP1-T70 and Anti-phospho-S6K Thr412 antibodies were obtained from Cell Signaling...

WebPhospho-4E-BP1 (Ser65) Antibody detects endogenous levels of 4E-BP1 when phosphorylated at serine 65. This antibody has been shown to also recognize 4E-BP1 when phosphorylated at serine 101 (See application reference, Wang et al. 2003). Species … WebProduct name Anti-eIF4EBP1 (phospho T70) antibody [EPR654 (2)A] See all eIF4EBP1 primary antibodies Description Rabbit monoclonal [EPR654 (2)A] to eIF4EBP1 (phospho T70) Host species Rabbit Tested applications …

WebRabbit anti 4EBP1 (pSer65) antibody recognizes eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) also known as phosphorylated heat-and acid-stable protein regulated by insulin 1 (PHAS-I), when phosphorylated at serine 65. 4EBP1 belongs to the eIF4E-binding protein family. 4EBP1, when bound to eIF4E, inhibits cap-dependent ... WebJun 25, 2010 · Anti-phospho Drosophila S6K, anti-S6K, anti-phospho S6K, anti-Akt, anti-phospho Akt, and anti-phospho 4EBP1 antibodies were from Cell Signaling. Anti-Myc, anti-HA, and anti-FLAG antibodies were from Santa Cruz Biotechnology, Covance, and Sigma, respectively. ... stimulation for 30 min. Phosphorylation and protein levels of dS6K were …

WebFeb 10, 2024 · Cell Signaling Technology's Phospho-4E-BP1 (Thr70) Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Precipitation, Immunoprecipitation, and Western Blot. Phospho-4E-BP1 (Thr70) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70.

Web4EBP1 is a 118 amino acid protein with a predicted molecular weight of 13 kD. Distribution Cytosol and nucleus/ubiquitously expressed Function Regulation of global translation Biology Area Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction Molecular Family Phospho-Proteins Antigen References raw material crisisWebEach 4EBP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our 4EBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. raw material color code chartWebSep 7, 2024 · 4E-BP1 Antibody (P-1) is recommended for detection of 4E-BP1 of mouse, rat, human and porcine origin by WB, IP, IF, IHC (P) and ELISA. Anti-4E-BP1 Antibody (P-1) is available conjugated to agarose for IP; HRP for WB, IHC (P) and ELISA; and to either phycoerythrin or FITC for IF, IHC (P) and FCM. raw material consumption sheetWebPhospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb simple homemade sushi rollsWebFigure 3: Anti-4E-BP1 (phospho Ser65 + Thr70) Antibody (AF2308) Western blot analysis of extracts from 293 cells treated with 20% FBS , using Phospho-4EBP1(Ser65/Thr70) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide. raw material costing raw material crosswordWebDot blot analysis of eIF4EBP1(phospho S65) using ab259329 at 1/1000 (0.522 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. Lane 1: eIF4EBP1 (phospho S65) peptide. … raw material cost inflation